Quantitative Microbial Risk Assessment (QMRA) methodology, which has already been applied to drinking water and food safety, may also be applied to risk assessment and management at the workplace. The present study developed a preliminary QMRA model to assess microbial risk that is associated with inhaling bioaerosols that are contaminated with human adenovirus (HAdV). This model has been applied to air contamination data from different occupational settings, including wastewater systems, solid waste landfills, and toilets in healthcare settings and offices, with different exposure times. Virological monitoring showed the presence of HAdVs in all the evaluated settings, thus confirming that HAdV is widespread, but with different average concentrations of the virus. The QMRA results, based on these concentrations, showed that toilets had the highest probability of viral infection, followed by wastewater treatment plants and municipal solid waste landfills. Our QMRA approach in occupational settings is novel, and certain caveats should be considered. Nonetheless, we believe it is worthy of further discussions and investigations.
REFERENCE:
Carducci, Annalaura et al. “Quantitative Microbial Risk Assessment in Occupational Settings Applied to the Airborne Human Adenovirus Infection.” Ed. Andrew Watterson. International Journal of Environmental Research and Public Health 13.7 (2016): 733. PMC. Web. 18 Aug. 2016.
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jueves, 8 de septiembre de 2016
lunes, 5 de septiembre de 2016
Occupational health related concerns among surgeons
The surgeon’s daily workload renders him/her susceptible to a variety of the common work-related illness. They are exposed to a number of occupational hazards in their professional work. These hazards include sharp injuries, blood borne pathogens, latex allergy, laser plumes, hazardous chemicals, anesthetic gases, equipment hazards, static postures, and job related stressors. However, many pay little attention to their health, and neither do they seek the appropriate help when necessary. It is observed that occupational hazards pose a huge risk to the personal well-being of surgeons. As such, the importance of early awareness and education alongside prompt intervention is duly emphasized. Therefore, increased attention to the health, economic, personal, and social implications of these injuries is essential for appropriate management and future prevention. These risks are as great as any other occupational hazards affecting surgeons today. The time has come to recognize and address them.REFERENCE:
Memon, Anjuman Gul et al. “Occupational Health Related Concerns among Surgeons.” International Journal of Health Sciences 10.2 (2016): 279–291. Print.
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jueves, 1 de septiembre de 2016
Prevalence of Respiratory Protective Devices in U.S. Health Care Facilities
An online questionnaire was developed to explore respiratory protective device (RPD) prevalence in U.S. health care facilities. The survey was distributed to professional nursing society members in 2014 and again in 2015 receiving 322 and 232 participant responses, respectively. The purpose of this study was to explore if the emergency preparedness climate associated with Ebola virus disease changed the landscape of RPD use and awareness. Comparing response percentages from the two sampling time frames using bivariate analysis, no significant changes were found in types of RPDs used in health care settings. N95 filtering facepiece respirators continue to be the most prevalent RPD used in health care facilities, but powered air-purifying respirators are also popular, with regional use highest in the West and Midwest. Understanding RPD use prevalence could ensure that health care workers receive appropriate device trainings as well as improve supply matching for emergency RPD stockpiling.
REFERENCE:
Wizner, Kerri et al. “Prevalence of Respiratory Protective Devices in U.S. Health Care Facilities: Implications for Emergency Preparedness.” Workplace health & safety 64.8 (2016): 359–368. PMC. Web. 18 Aug. 2016.
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REFERENCE:
Wizner, Kerri et al. “Prevalence of Respiratory Protective Devices in U.S. Health Care Facilities: Implications for Emergency Preparedness.” Workplace health & safety 64.8 (2016): 359–368. PMC. Web. 18 Aug. 2016.
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lunes, 29 de agosto de 2016
Indications and the requirements for single-use medical gloves
Aim: While the requirements for single-use gloves for staff protection are clearly defined, the conventional medical differentiation between “sterile surgical gloves” used during surgical procedures and “single-use medical gloves” used in non-sterile medical areas does not adequately define the different requirements in these two areas of use. Sterilization of single-use medical gloves is not performed if sterility is not required; thus, another terminology must be found to identify the safety quality of non-sterile single-use medical gloves. Therefore, the labeling of such gloves should reflect this situation, by introducing the term “pathogen-free” single-use glove. The hygienic safety of such a glove would be attainable by ensuring aseptic manufacturing conditions during manufacturing and control of pathogen load of batch controls after fabrication. Proposed recommendation: Because single-use gloves employed in non-sterile areas come into contact not only with intact skin but also with mucous membranes, no potential pathogens should be detectable in 100 mL of rinse sample. In order to declare such gloves as pathogen-free we suggest absence of the indicator species S. aureus and E. coli. In addition, the total CFU count should be evaluated, since a high load indicates lack of optimal hygiene during the manufacturing process. Based on the requirements for potable water and findings obtained from investigations of the bacterial load of such gloves after manufacturing, the here suggested limit for the total bacterial count of <102 CFU/mL of rinse sample per glove seems realistic. Keywords: single-use medical gloves, indications, requirements, definitions, “germ-poor” single-use gloves, pathogen-free single-use gloves
REFERENCE:
Kramer, A., & Assadian, O. (2016). Indications and the requirements for single-use medical gloves. GMS Hygiene and Infection Control, 11, Doc01. http://doi.org/10.3205/dgkh000261
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viernes, 26 de agosto de 2016
Engineered nanomaterials: toward effective safety management in research laboratories
Background: It is still unknown which types of nanomaterials and associated doses represent an actual danger to humans and environment. Meanwhile, there is consensus on applying the precautionary principle to these novel materials until more information is available. To deal with the rapid evolution of research, including the fast turnover of collaborators, a user-friendly and easy-to-apply risk assessment tool offering adequate preventive and protective measures has to be provided.
Results: Based on new information concerning the hazards of engineered nanomaterials, we improved a previously developed risk assessment tool by following a simple scheme to gain in efficiency. In the first step, using a logical decision tree, one of the three hazard levels, from H1 to H3, is assigned to the nanomaterial. Using a combination of decision trees and matrices, the second step links the hazard with the emission and exposure potential to assign one of the three nanorisk levels (Nano 3 highest risk; Nano 1 lowest risk) to the activity. These operations are repeated at each process step, leading to the laboratory classification. The third step provides detailed preventive and protective measures for the determined level of nanorisk.
Conclusions: We developed an adapted simple and intuitive method for nanomaterial risk management in research laboratories. It allows classifying the nanoactivities into three levels, additionally proposing concrete preventive and protective measures and associated actions. This method is a valuable tool for all the participants in nanomaterial safety. The users experience an essential learning opportunity and increase their safety awareness. Laboratory managers have a reliable tool to obtain an overview of the operations involving nanomaterials in their laboratories; this is essential, as they are responsible for the employee safety, but are sometimes unaware of the works performed. Bringing this risk to a three-band scale (like other types of risks such as biological, radiation, chemical, etc.) facilitates the management for occupational health and safety specialists. Institutes and school managers can obtain the necessary information to implement an adequate safety management system. Having an easy-to-use tool enables a dialog between all these partners, whose semantic and priorities in terms of safety are often different.
REFERENCE:
Groso, Amela et al. “Engineered Nanomaterials: Toward Effective Safety Management in Research Laboratories.” Journal of Nanobiotechnology 14 (2016): 21. PMC. Web. 18 Aug. 2016.
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Results: Based on new information concerning the hazards of engineered nanomaterials, we improved a previously developed risk assessment tool by following a simple scheme to gain in efficiency. In the first step, using a logical decision tree, one of the three hazard levels, from H1 to H3, is assigned to the nanomaterial. Using a combination of decision trees and matrices, the second step links the hazard with the emission and exposure potential to assign one of the three nanorisk levels (Nano 3 highest risk; Nano 1 lowest risk) to the activity. These operations are repeated at each process step, leading to the laboratory classification. The third step provides detailed preventive and protective measures for the determined level of nanorisk.
Conclusions: We developed an adapted simple and intuitive method for nanomaterial risk management in research laboratories. It allows classifying the nanoactivities into three levels, additionally proposing concrete preventive and protective measures and associated actions. This method is a valuable tool for all the participants in nanomaterial safety. The users experience an essential learning opportunity and increase their safety awareness. Laboratory managers have a reliable tool to obtain an overview of the operations involving nanomaterials in their laboratories; this is essential, as they are responsible for the employee safety, but are sometimes unaware of the works performed. Bringing this risk to a three-band scale (like other types of risks such as biological, radiation, chemical, etc.) facilitates the management for occupational health and safety specialists. Institutes and school managers can obtain the necessary information to implement an adequate safety management system. Having an easy-to-use tool enables a dialog between all these partners, whose semantic and priorities in terms of safety are often different.
REFERENCE:
Groso, Amela et al. “Engineered Nanomaterials: Toward Effective Safety Management in Research Laboratories.” Journal of Nanobiotechnology 14 (2016): 21. PMC. Web. 18 Aug. 2016.
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miércoles, 24 de agosto de 2016
#UANL: 2º Taller "Control de Riesgos Biológicos en Laboratorios de Investigación"
2º Taller "Control de Riesgos Biológicos en Laboratorios de Investigación"
12 de Septiembre de 2016
Facultad de Ciencias Biológicas, UANL.
Ciudad Universitaria, San Nicolás de los Garza, Nuevo León.
=> DESCARGAR PROGRAMA PDF <=
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lunes, 22 de agosto de 2016
Use of Protective Gloves in Nail Salons in Manhattan, New York City
Objectives: Nail salon owners in New York City (NYC) are required to provide their workers with gloves and it is their responsibility to maintain healthy, safe working spaces for their employees. The purpose of this study was to determine the frequency with which nail salon workers wear protective gloves.
Methods: A Freedom of Information Law request was submitted to New York Department of State’s Division of Licensing Services for a full list of nail salons in Manhattan, NYC. A sample population of 800 nail salons was identified and a simple random sample (without replacement) of 30% (n=240) was selected using a random number generator. Researchers visited each nail salon from October to December of 2015, posing as a potential customer to determine if nail salon workers were wearing gloves.
Results: Among the 169 salons in which one or more workers was observed providing services, a total of 562 workers were observed. For 149 salons, in which one or more worker was observed providing services, none of the workers were wearing gloves. In contrast, in six of the salons observed, in which one or more workers was providing services, all of the workers (1 in 2 sites, 2 in 1 site, 3 in 2 sites, and 4 in 1 site) were wearing gloves. Almost three-quarters of the total number of workers observed (n=415, 73.8%) were not wearing gloves.
Conclusions: The findings of this study indicate that, despite recent media attention and legislation, the majority of nail salon workers we observed were not wearing protective gloves when providing services.
REFERENCE:
Basch, Corey et al. “Use of Protective Gloves in Nail Salons in Manhattan, New York City.” Journal of Preventive Medicine and Public Health 49.4 (2016): 249–251. PMC. Web. 18 Aug. 2016.
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Methods: A Freedom of Information Law request was submitted to New York Department of State’s Division of Licensing Services for a full list of nail salons in Manhattan, NYC. A sample population of 800 nail salons was identified and a simple random sample (without replacement) of 30% (n=240) was selected using a random number generator. Researchers visited each nail salon from October to December of 2015, posing as a potential customer to determine if nail salon workers were wearing gloves.
Results: Among the 169 salons in which one or more workers was observed providing services, a total of 562 workers were observed. For 149 salons, in which one or more worker was observed providing services, none of the workers were wearing gloves. In contrast, in six of the salons observed, in which one or more workers was providing services, all of the workers (1 in 2 sites, 2 in 1 site, 3 in 2 sites, and 4 in 1 site) were wearing gloves. Almost three-quarters of the total number of workers observed (n=415, 73.8%) were not wearing gloves.
Conclusions: The findings of this study indicate that, despite recent media attention and legislation, the majority of nail salon workers we observed were not wearing protective gloves when providing services.
REFERENCE:
Basch, Corey et al. “Use of Protective Gloves in Nail Salons in Manhattan, New York City.” Journal of Preventive Medicine and Public Health 49.4 (2016): 249–251. PMC. Web. 18 Aug. 2016.
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jueves, 18 de agosto de 2016
Performance analysis of exam gloves used for aseptic rodent surgery
Aseptic technique includes the use of sterile surgical gloves for survival surgeries in rodents to minimize the incidence of infections. Exam gloves are much less expensive than are surgical gloves and may represent a cost-effective, readily available option for use in rodent surgery. This study examined the effectiveness of surface disinfection of exam gloves with 70% isopropyl alcohol or a solution of hydrogen peroxide and peracetic acid (HP-PA) in reducing bacterial contamination. Performance levels for asepsis were met when gloves were negative for bacterial contamination after surface disinfection and sham 'exertion' activity. According to these criteria, 94% of HP-PA-disinfected gloves passed, compared with 47% of alcohol-disinfected gloves. In addition, the effect of autoclaving on the integrity of exam gloves was examined, given that autoclaving is another readily available option for aseptic preparation. Performance criteria for glove integrity after autoclaving consisted of: the ability to don the gloves followed by successful simulation of wound closure and completion of stretch tests without tearing or observable defects. Using this criteria, 98% of autoclaved nitrile exam gloves and 76% of autoclaved latex exam gloves met performance expectations compared with the performance of standard surgical gloves (88% nitrile, 100% latex). The results of this study support the use of HP-PA-disinfected latex and nitrile exam gloves or autoclaved nitrile exam gloves as viable cost-effective alternatives to sterile surgical gloves for rodent surgeries.
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REFERENCE:
LeMoine DM, et al. Performance analysis of exam gloves used for aseptic rodent surgery. J Am Assoc Lab Anim Sci. 2015 May;54(3):311-6.
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lunes, 15 de agosto de 2016
Gain-of-Function Research: Ethical Analysis
Gain-of-function (GOF) research involves experimentation that aims or is expected to (and/or, perhaps, actually does) increase the transmissibility and/or virulence of pathogens. Such research, when conducted by responsible scientists, usually aims to improve understanding of disease causing agents, their interaction with human hosts, and/or their potential to cause pandemics. The ultimate objective of such research is to better inform public health and preparedness efforts and/or development of medical countermeasures. Despite these important potential benefits, GOF research (GOFR) can pose risks regarding biosecurity and biosafety. In 2014 the administration of US President Barack Obama called for a "pause" on funding (and relevant research with existing US Government funding) of GOF experiments involving influenza, SARS, and MERS viruses in particular. With announcement of this pause, the US Government launched a "deliberative process" regarding risks and benefits of GOFR to inform future funding decisions-and the US National Science Advisory Board for Biosecurity (NSABB) was tasked with making recommendations to the US Government on this matter. As part of this deliberative process the National Institutes of Health commissioned this Ethical Analysis White Paper, requesting that it provide (1) review and summary of ethical literature on GOFR, (2) identification and analysis of existing ethical and decision-making frameworks relevant to (i) the evaluation of risks and benefits of GOFR, (ii) decision-making about the conduct of GOF studies, and (iii) the development of US policy regarding GOFR (especially with respect to funding of GOFR), and (3) development of an ethical and decision-making framework that may be considered by NSABB when analyzing information provided by GOFR risk-benefit assessment, and when crafting its final recommendations (especially regarding policy decisions about funding of GOFR in particular). The ethical and decision-making framework ultimately developed is based on the idea that there are numerous ethically relevant dimensions upon which any given case of GOFR can fare better or worse (as opposed to there being necessary conditions that are either satisfied or not satisfied, where all must be satisfied in order for a given case of GOFR to be considered ethically acceptable): research imperative, proportionality, minimization of risks, manageability of risks, justice, good governance (i.e., democracy), evidence, and international outlook and engagement. Rather than drawing a sharp bright line between GOFR studies that are ethically acceptable and those that are ethically unacceptable, this framework is designed to indicate where any given study would fall on an ethical spectrum-where imaginable cases of GOFR might range from those that are most ethically acceptable (perhaps even ethically praiseworthy or ethically obligatory), at one end of the spectrum, to those that are most ethically problematic or unacceptable (and thus should not be funded, or conducted), at the other. The aim should be that any GOFR pursued (and/or funded) should be as far as possible towards the former end of the spectrum.
REFERENCE:
Selgelid MJ. Gain-of-Function Research: Ethical Analysis. Sci Eng Ethics. 2016 Aug 8. doi:10.1007/s11948-016-9810-1
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REFERENCE:
Selgelid MJ. Gain-of-Function Research: Ethical Analysis. Sci Eng Ethics. 2016 Aug 8. doi:10.1007/s11948-016-9810-1
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viernes, 12 de agosto de 2016
Soliciting Stakeholder Input for a Revision of Biosafety in Microbiological and Biomedical Laboratories (BMBL): Proceedings of a Workshop.
Since its publication by the National Institutes of Health (NIH) and the Centers for Disease Control and Prevention (CDC) in 1984, Biosafety in Microbiological and Biomedical Laboratories (BMBL) has become the cornerstone of the practice of biosafety in the United States and in many countries around the world. The BMBL has been revised periodically over the past three decades to refine the guidance it provides based on new knowledge and experiences—allowing it to remain a relevant, valuable, and authoritative reference for the microbiological and biomedical community. Seven years after the release of the BMBL 5th Edition, NIH and CDC are considering a revision based on the comments of a broader set of stakeholders. At the request of NIH, the National Academies of Sciences, Engineering and Medicine conducted a virtual town hall meeting from 4 April to 20 May 2016 to allow BMBL users to share their thoughts on the BMBL in general and its individual sections and appendices. Specifically, users were asked to indicate what information they think should be added, revised, or deleted. Major themes from the virtual town hall meeting were further discussed in a workshop held on 12 May 2016 in Washington, DC. This document encapsulates the discussion of the major comments on the BMBL that were posted on the virtual town hall prior to 12 May 2016 and the various BMBL comments and issues related to biosafety that were raised during the workshop by participants who attended the meeting in Washington DC and those who listened to the live webcast.
REFERENCE:
Board on Agriculture and Natural Resources; Division on Earth and Life Studies; National Academies of Sciences, Engineering, and Medicine. Soliciting Stakeholder Input for a Revision of Biosafety in Microbiological and Biomedical Laboratories (BMBL): Proceedings of a Workshop. WASHINGTON (DC): National Academies Press (US); 2016 Jul.
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REFERENCE:
Board on Agriculture and Natural Resources; Division on Earth and Life Studies; National Academies of Sciences, Engineering, and Medicine. Soliciting Stakeholder Input for a Revision of Biosafety in Microbiological and Biomedical Laboratories (BMBL): Proceedings of a Workshop. WASHINGTON (DC): National Academies Press (US); 2016 Jul.
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viernes, 29 de julio de 2016
Interim Guidance for Health Care Providers Caring for Pregnant Women with Possible #Zika Virus Exposure
CDC has updated its interim guidance for U.S. health care providers caring for pregnant women with possible Zika virus exposure, to include the emerging data indicating that Zika virus RNA can be detected for prolonged periods in some pregnant women. To increase the proportion of pregnant women with Zika virus infection who receive a definitive diagnosis, CDC recommends expanding real-time reverse transcription–polymerase chain reaction (rRT-PCR) testing. Possible exposures to Zika virus include travel to or residence in an area with active Zika virus transmission, or sex* with a partner who has traveled to or resides in an area with active Zika virus transmission without using condoms or other barrier methods to prevent infection.† Testing recommendations for pregnant women with possible Zika virus exposure who report clinical illness consistent with Zika virus disease§ (symptomatic pregnant women) are the same, regardless of their level of exposure (i.e., women with ongoing risk for possible exposure, including residence in or frequent travel to an area with active Zika virus transmission, as well as women living in areas without Zika virus transmission who travel to an area with active Zika virus transmission, or have unprotected sex with a partner who traveled to or resides in an area with active Zika virus transmission). Symptomatic pregnant women who are evaluated <2 weeks after symptom onset should receive serum and urine Zika virus rRT-PCR testing. Symptomatic pregnant women who are evaluated 2–12 weeks after symptom onset should first receive a Zika virus immunoglobulin (IgM) antibody test; if the IgM antibody test result is positive or equivocal, serum and urine rRT-PCR testing should be performed. Testing recommendations for pregnant women with possible Zika virus exposure who do not report clinical illness consistent with Zika virus disease (asymptomatic pregnant women) differ based on the circumstances of possible exposure. For asymptomatic pregnant women who live in areas without active Zika virus transmission and who are evaluated <2 weeks after last possible exposure, rRT-PCR testing should be performed. If the rRT-PCR result is negative, a Zika virus IgM antibody test should be performed 2–12 weeks after the exposure. Asymptomatic pregnant women who do not live in an area with active Zika virus transmission, who are first evaluated 2–12 weeks after their last possible exposure should first receive a Zika virus IgM antibody test; if the IgM antibody test result is positive or equivocal, serum and urine rRT-PCR should be performed. Asymptomatic pregnant women with ongoing risk for exposure to Zika virus should receive Zika virus IgM antibody testing as part of routine obstetric care during the first and second trimesters; immediate rRT-PCR testing should be performed when IgM antibody test results are positive or equivocal. This guidance also provides updated recommendations for the clinical management of pregnant women with confirmed or possible Zika virus infection. These recommendations will be updated when additional data become available.
REFERENCE:
Oduyebo T, et al. Update: Interim Guidance for Health Care Providers Caring for Pregnant Women with Possible Zika Virus Exposure — United States, July 2016. MMWR Morb Mortal Wkly Rep 2016;65:739–744. DOI: http://dx.doi.org/10.15585/mmwr.mm6529e1
Other:
Brooks JT, et al. Update: Interim Guidance for Prevention of Sexual Transmission of Zika Virus — United States, July 2016. MMWR Morb Mortal Wkly Rep 2016;65:745–747. DOI: http://dx.doi.org/10.15585/mmwr.mm6529e2
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REFERENCE:
Oduyebo T, et al. Update: Interim Guidance for Health Care Providers Caring for Pregnant Women with Possible Zika Virus Exposure — United States, July 2016. MMWR Morb Mortal Wkly Rep 2016;65:739–744. DOI: http://dx.doi.org/10.15585/mmwr.mm6529e1
Other:
Brooks JT, et al. Update: Interim Guidance for Prevention of Sexual Transmission of Zika Virus — United States, July 2016. MMWR Morb Mortal Wkly Rep 2016;65:745–747. DOI: http://dx.doi.org/10.15585/mmwr.mm6529e2
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jueves, 28 de julio de 2016
HISTORY 1951: Yellow fever and Max Theiler: the only Nobel Prize for a virus vaccine
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| Max Theiler receives the Nobel Prize in Physiology or Medicine from the hands of His Majesty the King Gustaf Adolf VI on December 10, 1951. Photo provided by the Karolinska Institutet. |
REFERENCE:
Norrby, Erling. “Yellow Fever and Max Theiler: The Only Nobel Prize for a Virus Vaccine.” The Journal of Experimental Medicine 204.12 (2007): 2779–2784. PMC. Web. 27 July 2016.
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miércoles, 27 de julio de 2016
Día Mundial contra la Hepatitis, Julio 28 / #Hepatitis world day, July 28th #NoHep
Únase a ésta campaña: http://worldhepatitisday.org/es
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miércoles, 20 de julio de 2016
Hipoclorito de sodio como agente desinfectante
Este artículo es sobre soluciones desinfectantes, siga el link para ver:
Publicado originalmente el 19 de Julio de 2008.
Actualizado 03/feb/2025
El hipoclorito de sodio (NaOCl) es un compuesto oxidante de rápida acción utilizado a gran escala para la desinfección de superficies, desinfección de ropa hospitalaria y desechos, descontaminar salpicaduras de sangre, desinfección de equipos y mesas de trabajo resistentes a la oxidación, eliminación de olores y desinfección del agua. Los equipos o muebles metálicos tratados con cloro, tienden a oxidarse rápidamente en presencia de hipoclorito de sodio.
El hipoclorito de sodio es vendido en una solución clara de ligero color verde-amarillento y un olor característico. Como agente blanqueante de uso domestico normalmente contiene 5-6.5% de hipoclorito de sodio (con un pH de alrededor de 11, es irritante y corrosivo a los metales). Cuando el hipoclorito se conserva en su contenedor a temperatura ambiente y sin abrirlo, puede conservarse durante 1 mes, pero cuando se ha utilizado para preparar soluciones, se recomienda su cambio diario. Entre sus muchas propiedades incluyen su amplia y rápida actividad antimicrobiana, relativa estabilidad, fácil uso y bajo costo.
El hipoclorito es letal para varios microorganismos, virus y bacterias vegetativas, pero es menos efectivo contra esporas bacterianas, hongos y protozoarios. La actividad del hipoclorito se ve reducida en presencia de iones metálicos, biocapas, materiales orgánicos, bajo pH o luz UV. Las soluciones de trabajo deben ser preparadas diariamente. El cloro comercial que contiene 5-6%, que será utilizado para la desinfección de superficies, debe ser diluído 1:10 para obtener una concentración final de aproximadamente 0.5% de hipoclorito. Cuando se quiere desinfectar líquidos que pueden contener material orgánico, debe tenerse una concentración final de 1% de hipoclorito.
Gracias a su alta disponibilidad continua siendo de alto uso en hospitales. Pueden encontrar otras características y hojas de seguridad del hipoclorito de sodio.
El modo de acción del hipoclorito es la oxidación: oxida proteínas, oxida DNA y RNA, oxida grasas, oxida metales.. OXIDA!, OXIDA!, OXIDA!, OXIDA!, OXIDA!...
RECOMENDACIONES PARA LA PREPARACIÓN Y USO:
#VIDEOBLOG:
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CONCENTRACIONES RECOMENDADAS
- Venta al público: (Blanqueador casero, presentación comercial): 5-6 % (50-60 g/l, 50,000 ppm) de cloro libre
- Para desinfección con material orgánico o derrames: 1% (10 g/l, 10,000 ppm)
- Para desinfección general de áreas sin materia orgánica: 0.5% (5g/L; 5,000 ppm)
- Para desinfección de superficies (CORONAVIRUS): 0.2%
- Para limpieza general, desinfección de manos, desinfección de ropa: 0.05% (500 mg/L; 500 ppm) *
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RECOMENDACIONES PARA LA PREPARACIÓN Y USO:
- Antes de elegir un agente desinfectante, por favor revisa su efectividad para el microorganismo que te interesa.
- USAR agua destilada o desinizada. El agua de la llave contiene muchos metales y sales que interfieren con su efectividad.
- Revisar la etiqueta antes de preparar el hipoclorito de sodio buscando la caducidad y la concentración de venta.
- Existen dos tipos de hipoclorito de sodio. El regular, que tiene una caducidad de 2 a 3 meses, y el "estabilizado", que tiene una caducidad de 1 a 2 años. Pero ambos se degradan rápidamente una vez preparados, por lo que no deben utilizarse después de 5 días de su preparación.
- Almacenar en un lugar fresco, seco y obscuro, ya que la luz y el calor aceleran su degradación.
- Existen varios procedimientos para la desinfección, por ejemplo LAVADO => DESINFECCIÓN => ENJUAGUE, es decir, realizar un lavado antes de la desinfección para retirar materia orgánica, luego aplicar el desinfectante, y realizar enjuagado para eliminar el exceso de desinfectate.
- Para la desinfección de líquidos que puedan contener microorganismos, debe prepararse una solución al 2% de hipoclorito de sodio. Posteriormente, mezclar en proporción 1:1 (1 volumen de desinfectante, 1 volumen de líquido). De esta forma, al final tendrá una concentración de 1%. Dejar reposar durante 30 minutos. Por ejemplo: 200 ml de orina + 200 ml de solución de hipoclorito de sodio al 2%.
- Para desinfectar superficies o materiales de laboratorio (que no sean metálicos), que no contengan material orgánico, deberá usarse una solución de hipoclorito de sodio al 0.5%. Por ejemplo, para desinfectar gradillas de laboratorio de plástico, sumérjalas en la solución al 0.5% por al menos 30 minutos.
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FÓRMULA PARA PREPARAR SOLUCIONES DESINFECTANTES
Cualquier concentración puede ser utilizada para obtener una solución de hipoclorito diluída utilizando la siguiente fórmula: =>
Por ejemplo para preparar una solución 0.5% a partir de una 4.5% de hipoclorito de sodio se utilizarán 8 partes de agua con 1 parte de hipoclorito de sodio.
Donde "parte" puede ser utilizado para cualquier unidad de medida (litro, mililitro, galones, etc), o utilizando cualquier medidor (taza, frasco, garrafón, etc). En países de habla francesa, la cantidad de hipoclorito se expresa como "grados de cloro". Un grado de cloro = 0.3% de cloro activo. (Ref. 8)
Otra fórmula para calcular el volumen necesario para preparar el hipoclorito de sodio 0.5% a partir de una solución concentrada:
PREPARACIÓN RÁPIDA DE HIPOCLORITO DE SODIO
DESINFECCIÓN DE SÁBANAS Y ROPA DE CAMA Otra fórmula para calcular el volumen necesario para preparar el hipoclorito de sodio 0.5% a partir de una solución concentrada:
REVISAR LA ETIQUETA PARA VER LA CONCENTRACIÓN DE CLORO
DESCARGAR AQUI TABLA PARA PREPARAR HIPOCLORITO DE SODIO CON FINES DE DESINFECCIÓN PDF
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PREPARACIÓN RÁPIDA DE HIPOCLORITO DE SODIO
En el caso de coronavirus COVID-19 las concentraciones consideradas efectivas es a partir del 0.2%. El tiempo de contacto recomendado es de 2 a 5 minutos.
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Toda la ropa de cama que ha estado en contacto con pacientes puede estar contaminado con líquidos o fluidos corporales (orina, sangre, vómito). Cuando se manejan este tipo de ropa, debe utilizarse equipo de protección adecuado, pero debe incluirse, guantes, mascarillas, lentes de protección, batas y botas. Los excesos de excremento deberán retirarse y colocarse en bolsas para desechos. Antes de desinfectar, deberá realizarse un lavado en lavadora con agua y jabón. Enjuagar para eliminar el exceso de jabón. Finalmente, colocar las sábanas en una solución de hipoclorito de sodio al 0.05%, durante por lo menos 30 minutos ó una hora. Puede realizarse un segundo enjuague para eliminar el exceso de hipoclorito, y continuar con los procesos normales de secado.
El lavado a mano debe evitarse en la medida de lo posible. Cuando por las condiciones, no puede utilizarse lavadoras automáticas, las sábanas deberán colocarse en un gran contenedor con agua caliente y jabón, y agitar en círculos con un palo o varilla. Eliminar el agua, y colocar una solución al 0.1% de hipoclorito de sodio por 15 minutos, sumergiendo completamente las sábanas. Enjuagar nuevamente y dejar secar, evitando sacudir en la medida de lo posible (Ver Ref. 8).
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SOBRE LA INESTABILIDAD DEL CLORO:
Una vez preparadas, las soluciones comunes de hipoclorito de sodio guardadas a 25ºC, en recipientes cerrados, contenedores opacos, pierden 50% de su contenido de cloro libre en un periodo de 30 días. Una solución al 1%, tendrá solo 0.5% de cloro 30 días después de preparado. Las soluciones al 5% se degradan más lentamente si se almacenan en contenedores obscuros. A mayor temperatura y con mayor cantidad de luz que reciban, el proceso de degradación se acelera (Ref. 6).
Existen soluciones "estabilizadas" de hipoclorito de sodio, que tienen una caducidad mínima de 1 año. Estas soluciones deben mantenerse a menos de 25ºC, lejos de la luz del sol y son comercializadas con ese nombre de "estabilizadas". Estas soluciones se mantienen estables mientras se encuentran bien cerradas en su envase original, ya que una vez que se preparan soluciones a partir de ellas, comienza su proceso de rápida degradación, debido a que los "estabilizadores" se diluyen. El hipoclorito de sodio normal se degrada rápidamente (Ref. 11).
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SOBRE LA TOXICIDAD DEL CLORO:
El hipoclorito de sodio ocasiona:
- Irritación ocular, orofaríngea, esofagial y quemaduras gástricas.
- Corrosión a los metales
- Reacciona de forma tóxica con el amoniaco y ácidos (presente en los productos desinfectantes comunes), por lo que no deben hacerse mezclas de desinfectantes.
- Producción de carcinógeno bis (clorometil) eter cuando se mezcla con formaldehído.
- Producción de carcinógeno trihalometano cuando el agua es hiperclorinada (exceso de cloro).
- Para la potabilización del agua, la NOM-127-SSA1-2021, establece que debe vigilarse los residos producto de la clorinación tales como: [1] Cloro residual libre, tabla 9; [2] trihalometanos (Bromodiclorometano, Bromoformo, Cloroformo y Dibromoclorometano), tabla 10, [3] ácidos haloacéticos (Ácido cloroacético, Ácido dicloroacético, Ácido tricloroacético), tabla 11.
Por favor visite esta página para ver las características y tratamiento de la intoxicación por cloro: https://medlineplus.gov/spanish/ency/article/002772.htm
WEBINAR: Toxicidad del Dióxido de Cloro
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REFERENCIAS:
- Rutala WA and Weber DJ. Uses of Inorganic Hypochlorite (Bleach) in Health-Care Facilities. Clinical Microbiological Reviews 1997; 10(4):597-610. PDF.
- Enviromental Health and Safety. University of Kentucky. PDF.
- Sil, T., Malyshev, D., Aspholm, M. et al. Boosting hypochlorite’s disinfection power through pH modulation. BMC Microbiol 25, 101 (2025). https://doi.org/10.1186/s12866-025-03831-w
- Uso de desinfectantes. Guías para la prevención, control y vigilancia epidemiológica de infecciones intrahospitalarias. Secretaría Distrital de Salud de Bogotá. PDF.
- Githui WA, Matu SW, Tunge N, Juma E. Biocidal effect of bleach on Mycobacterium tuberculosis: a safety measure. Int J Tuberc Lung Dis 2007. 11(7):798–802. PDF.
- Hojas de seguridad de microorganismos, con las recomendaciones de agentes desinfectantes.
- Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008. CDC.
- Intoxicación con hipoclorito de sodio
- How to make chlorine solutions for environmental disinfection (Annex 6 from Interim Infection Prevention and Control Guidance for Care of Patients with Suspected or Confirmed Filovirus Haemorrhagic Fever in Health-Care Settings, with Focus on Ebola 2014)
- OSHA: Cleaning and Decontamination of #Ebola on Surfaces. Guidance for Workers and Employers in Non-Healthcare/Non-Laboratory Settings
- For General Healthcare Settings in West Africa: How to Prepare and Use Chlorine Solutions
- D. Lantagne, et al. Hypochlorite Solution Expiration and Stability in Household Water Treatment in Developing Countries. Journal of Environmental Engineering, Vol. 137, No. 2, February 1, 2011.
- Wolfe, Marlene K et al. “Handwashing and Ebola virus disease outbreaks: A randomized comparison of soap, hand sanitizer, and 0.05% chlorine solutions on the inactivation and removal of model organisms Phi6 and E. coli from hands and persistence in rinse water” PloS one vol. 12,2 e0172734. 23 Feb. 2017, doi:10.1371/journal.pone.0172734
- Potential role of inanimate surfaces for the spread ofcoronaviruses and their inactivation with disinfectantagents
- Kampf, G et al. Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents. The Journal of hospital infection vol. 104,3 (2020): 246-251. doi:10.1016/j.jhin.2020.01.022
- Lai, Mary Y Y et al. Survival of severe acute respiratory syndrome coronavirus. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America vol. 41,7 (2005): e67-71. doi:10.1086/433186
- Lai, Mary Y Y et al. Survival of severe acute respiratory syndrome coronavirus. Clinical infectious diseases.vol. 41,7 (2005): e67-71. doi:10.1086/433186.
- Hulkower, Rachel L et al. “Inactivation of surrogate coronaviruses on hard surfaces by health care germicides.” American journal of infection control vol. 39,5 (2011): 401-407. doi:10.1016/j.ajic.2010.08.011
- NORMA Oficial Mexicana NOM-127-SSA1-2021, Agua para uso y consumo humano. Límites permisibles de la calidad del agua. https://www.dof.gob.mx/nota_detalle.php?codigo=5650705&fecha=02/05/2022#gsc.tab=0
- Guidelines for drinking-water quality (WHO). Fourth edition, incorporating the first and second addenda. https://iris.who.int/bitstream/handle/10665/352532/9789240045064-eng.pdf?sequence=1
lunes, 18 de julio de 2016
Marburg Virus Reverse Genetics Systems
The highly pathogenic Marburg virus (MARV) is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses. Reverse genetics systems established for MARV have been used to study various aspects of the viral replication cycle, analyze host responses, image viral infection, and screen for antivirals. This article provides an overview of the currently established MARV reverse genetic systems based on minigenomes, infectious virus-like particles and full-length clones, and the research that has been conducted using these systems.
Keywords: Marburg virus; Ebola virus; filoviruses; nonsegmented negative-sense RNA viruses; reverse genetics system; minigenome; full-length clones; virus-like particles; virus rescue; biosafety level 4
REFERENCE:
Schmidt KM, Mühlberger E. Marburg Virus Reverse Genetics Systems. Viruses. 2016 Jun 22;8(6).
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Keywords: Marburg virus; Ebola virus; filoviruses; nonsegmented negative-sense RNA viruses; reverse genetics system; minigenome; full-length clones; virus-like particles; virus rescue; biosafety level 4
REFERENCE:
Schmidt KM, Mühlberger E. Marburg Virus Reverse Genetics Systems. Viruses. 2016 Jun 22;8(6).
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jueves, 14 de julio de 2016
Molecular epidemiology and phylogeny of Nipah virus infection: A mini review.
Nipah virus (NiV) is a member of the genus Henipavirus of the family Paramyxoviridae, characterized by high pathogenicity and endemic in South Asia. It is classified as a Biosafety Level-4 (BSL-4) agent. The case-fatality varies from 40% to 70% depending on the severity of the disease and on the availability of adequate healthcare facilities. At present no antiviral drugs are available for NiV disease and the treatment is just supportive. Phylogenetic and evolutionary analyses can be used to help in understanding the epidemiology and the temporal origin of this virus. This review provides an overview of evolutionary studies performed on Nipah viruses circulating in different countries. Thirty phylogenetic studies have been published from 2000 to 2015 years, searching on pub-med using the key words 'Nipah virus AND phylogeny' and twenty-eight molecular epidemiological studies from 2006 to 2015 have been performed, typing the key words 'Nipah virus AND molecular epidemiology'. Overall data from the published study demonstrated as phylogenetic and evolutionary analysis represent promising tools to evidence NiV epidemics, to study their origin and evolution and finally to act with effective preventive measure.
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REFERENCE:
- Angeletti S, et al. Molecular epidemiology and phylogeny of Nipah virus infection: A mini review. Asian Pac J Trop Med. 2016 Jul;9(7):630-4. doi: 10.1016/j.apjtm.2016.05.012. Epub 2016 May 31. Review. PubMed PMID: 27393089.
- Nipah virus: Pathogen Safety Data Sheet
- ¿Qué es la enfermedad causada por el virus Nipah?
lunes, 11 de julio de 2016
Use of RODAC plates to measure containment of Mycobacterium #tuberculosis in a Class IIB biosafety cabinet during routine operations.
OBJECTIVE/BACKGROUND: Guidelines for the manipulation of Mycobacterium tuberculosis (MTB) cultures require a Biosafety Level 3 (BSL-3) infrastructure and accompanying code of conduct. In this study, we aimed to validate and apply detection methods for viable mycobacteria from surfaces in a BSL-3 MTB laboratory.
METHODS: We evaluated phenotypic (Replicate Organism Detection and Counting [RODAC] plates) and molecular (propidium monoazide [PMA]-based polymerase chain reaction [PCR]) approaches for the detection of viable mycobacteria, as well as the effect of 70% ethanol applied for 5min for disinfection against mycobacteria. For validation of the method, recovery of serial dilutions of Mycobacterium bovis bacillus Calmette-Guérin from glass slides was measured. Subsequently, we stamped surfaces in and around the biosafety cabinet (BSC) after different technicians had manipulated high bacterial load suspensions for routine drug-susceptibility testing in a Class II BSC.
RESULTS: RODAC stamping could detect as few as three bacteria on slides stamped either 5min or 60min after inoculation. PMA-based PCR, tested in parallel, did not pass validation. Mycobacteria were still detected after 5-min disinfection with ethanol 70%. In the BSL-3, from 201 RODAC-stamped surfaces, MTB was detected in four: three inside a BSC-on a tube cap and on an operator's gloves-and one outside, on an operator's gown.
CONCLUSION: RODAC plates detect mycobacteria at low numbers of microorganisms. In addition, this method allowed us to show that 70% ethanol does not reliably kill mycobacteria when applied for 5min to a dried surface, and that MTB bacilli may arrive outside a Class II BSC during routine practice, although the route could not be documented.
KEYWORDS: Biosafety; Environmental sampling; Ethanol; Propidium monoazide; Replicate Organism Detection and Counting; Tuberculosis
REFERENCE:
Daneau G, et al. Use of RODAC plates to measure containment of Mycobacterium tuberculosis in a Class IIB biosafety cabinet during routine operations. Int J Mycobacteriol. 2016 Jun;5(2):148-54.
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METHODS: We evaluated phenotypic (Replicate Organism Detection and Counting [RODAC] plates) and molecular (propidium monoazide [PMA]-based polymerase chain reaction [PCR]) approaches for the detection of viable mycobacteria, as well as the effect of 70% ethanol applied for 5min for disinfection against mycobacteria. For validation of the method, recovery of serial dilutions of Mycobacterium bovis bacillus Calmette-Guérin from glass slides was measured. Subsequently, we stamped surfaces in and around the biosafety cabinet (BSC) after different technicians had manipulated high bacterial load suspensions for routine drug-susceptibility testing in a Class II BSC.
RESULTS: RODAC stamping could detect as few as three bacteria on slides stamped either 5min or 60min after inoculation. PMA-based PCR, tested in parallel, did not pass validation. Mycobacteria were still detected after 5-min disinfection with ethanol 70%. In the BSL-3, from 201 RODAC-stamped surfaces, MTB was detected in four: three inside a BSC-on a tube cap and on an operator's gloves-and one outside, on an operator's gown.
CONCLUSION: RODAC plates detect mycobacteria at low numbers of microorganisms. In addition, this method allowed us to show that 70% ethanol does not reliably kill mycobacteria when applied for 5min to a dried surface, and that MTB bacilli may arrive outside a Class II BSC during routine practice, although the route could not be documented.
KEYWORDS: Biosafety; Environmental sampling; Ethanol; Propidium monoazide; Replicate Organism Detection and Counting; Tuberculosis
REFERENCE:
Daneau G, et al. Use of RODAC plates to measure containment of Mycobacterium tuberculosis in a Class IIB biosafety cabinet during routine operations. Int J Mycobacteriol. 2016 Jun;5(2):148-54.
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jueves, 7 de julio de 2016
#CRISPR, the disruptor
By Heidi Ledford
A powerful gene-editing technology is the biggest game changer to hit biology since PCR. But with its huge potential come pressing concerns.
By and large, researchers see these gaps as a minor price to pay for a powerful technique. But Doudna has begun to have more serious concerns about safety. Her worries began at a meeting in 2014, when she saw a postdoc present work in which a virus was engineered to carry the CRISPR components into mice. The mice breathed in the virus, allowing the CRISPR system to engineer mutations and create a model for human lung cancer4. Doudna got a chill; a minor mistake in the design of the guide RNA could result in a CRISPR that worked in human lungs as well. “It seemed incredibly scary that you might have students who were working with such a thing,” she says. “It's important for people to appreciate what this technology can do.”
REFERENCE:
Nature 522, 20–24 (04 June 2015) doi:10.1038/522020a
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A powerful gene-editing technology is the biggest game changer to hit biology since PCR. But with its huge potential come pressing concerns.
By and large, researchers see these gaps as a minor price to pay for a powerful technique. But Doudna has begun to have more serious concerns about safety. Her worries began at a meeting in 2014, when she saw a postdoc present work in which a virus was engineered to carry the CRISPR components into mice. The mice breathed in the virus, allowing the CRISPR system to engineer mutations and create a model for human lung cancer4. Doudna got a chill; a minor mistake in the design of the guide RNA could result in a CRISPR that worked in human lungs as well. “It seemed incredibly scary that you might have students who were working with such a thing,” she says. “It's important for people to appreciate what this technology can do.”
REFERENCE:
Nature 522, 20–24 (04 June 2015) doi:10.1038/522020a
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martes, 5 de julio de 2016
Inactivation and Environmental Stability of #Zika Virus
Working with Zika virus, a Biosafety Level 2 (BSL-2) pathogen in the European Union, except for the United Kingdom (where it is BSL-3), requires specific safety precautions. No inactivation data specific for Zika virus are available; consequently, disinfection guidelines are based on protocols to inactivate other flaviviruses. To gain experimental evidence regarding inactivation and disinfection for Zika virus, we determined its susceptibility to various disinfectants and inactivation methods.
REFERENCES:
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REFERENCES:
- Müller JA, et al. Inactivation and environmental stability of Zika virus. Emerg Infect Dis. 2016 Sep [Accesed Jul 5th, 2016]
- Technical Appendix
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lunes, 4 de julio de 2016
Precauciones para trabajadores de la salud contra la exposición a virus #Zika, en salas de parto
Los CDC recomiendan tomar precauciones estándar en todos los entornos de atención médica para evitar que el personal y los pacientes se infecten con el virus del Zika y patógenos a través de la sangre (por ej., el virus de la inmunodeficiencia humana [VIH] y el virus de la hepatitis C [VHC]). Debido al riesgo de exposición a grandes cantidades de líquidos corporales durante el trabajo de parto y a la naturaleza impredecible y apresurada de la atención obstétrica, es fundamental tomar medidas de precaución estándar en dichos entornos para evitar la transmisión del virus del Zika de pacientes infectados al personal de atención médica.
REFERENCIAS:
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REFERENCIAS:
- Needle stick infects lab worker with Zika virus
- Olson CK, et al. Preventing Transmission of Zika Virus in Labor and Delivery Settings Through Implementation of Standard Precautions - United States, 2016. MMWR Morb Mortal Wkly Rep 2016;65:290–292.
- FactSheet: Interim Guidance for Protecting Workers from Occupational Exposure to Zika Virus.
- Oduyebo T, et al. Update: Interim Guidelines for Health Care Providers Caring for Pregnant Women and Women of Reproductive Age with Possible Zika Virus Exposure — United States, 2016. MMWR Morb Mortal Wkly Rep 2016;65:122–127.
- NYCOSH Factsheet: What healthcare workers need to know about Zika Virus.
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jueves, 30 de junio de 2016
Guide to Implementing a Safety Culture in our Universities
This guide is a roadmap for a university-wide effort to strengthen a culture of research safety. The guide has action steps, resources, and recommendations to help navigate the challenge of changing the culture of the institution. The guide is intended for university presidents and chancellors who have made a renewed commitment to improve their institutional culture of safety, and it is intended for the campus leadership team that the president appoints to helm this effort. The task force encourages each institution to use the guide in ways that fits their unique institutional contexts. The task force has identified 20 recommendations for implementing and sustaining a culture of academic and research safety, primarily drawn from four foundational reports: Safe Science: Promoting a Culture of Safety in Academic Chemical Research (National Research Council, 2014); Creating Safety Cultures in Academic Institutions (ACS, 2012); Creating a Safety Culture (OSHA, 1989); and Texas Tech Laboratory Explosion Case Study (CSB, 2010). This guide includes an analysis showing the alignment of the 20 recommendations with these key national reports.
The recommendations are organized in four overarching categories: institution-wide dynamics and resources; data, hazard identification, and analysis; training and learning; and continuous improvement. For each recommendation, the task force has provided reading lists, tools, strategies, illustrative examples, and/or best practices drawn from a community of stakeholders for implementing these recommendations. These resources were selected to help an appointed campus team navigate the process of strengthening their culture of safety.
REFERENCE:
APLU Council on Research Task Force on Laboratory Safety (2016). A Guide to Implementing
a Safety Culture in Our Universities. CoR Paper 1. Washington, DC: Association of Public and
Land-grant Universities.
lunes, 27 de junio de 2016
The Respiratory Protection Effectiveness Clinical Trial (ResPECT)
BACKGROUND: Although N95 filtering facepiece respirators and medical masks are commonly used for protection against respiratory infections in healthcare settings, more clinical evidence is needed to understand the optimal settings and exposure circumstances for healthcare personnel to use these devices. A lack of clinically germane research has led to equivocal, and occasionally conflicting, healthcare respiratory protection recommendations from public health organizations, professional societies, and experts.
METHODS: The Respiratory Protection Effectiveness Clinical Trial (ResPECT) is a prospective comparison of respiratory protective equipment to be conducted at multiple U.S. study sites. Healthcare personnel who work in outpatient settings will be cluster-randomized to wear N95 respirators or medical masks for protection against infections during respiratory virus season. Outcome measures will include laboratory-confirmed viral respiratory infections, acute respiratory illness, and influenza-like illness. Participant exposures to patients, coworkers, and others with symptoms and signs of respiratory infection, both within and beyond the workplace, will be recorded in daily diaries. Adherence to study protocols will be monitored by the study team.
DISCUSSION: ResPECT is designed to better understand the extent to which N95s and MMs reduce clinical illness among healthcare personnel. A fully successful study would produce clinically relevant results that help clinician-leaders make reasoned decisions about protection of healthcare personnel against occupationally acquired respiratory infections and prevention of spread within healthcare systems.
TRIAL REGISTRATION: The trial is registered at clinicaltrials.gov, number NCT01249625 (11/29/2010).
KEYWORDS: Healthcare personnel; Influenza; Masks; Respirators; Respiratory infections
REFERENCE:
Radonovich LJ Jr, et al. The Respiratory Protection Effectiveness Clinical Trial (ResPECT): a cluster-randomized comparison of respirator and medical mask effectiveness against respiratory infections in healthcare personnel. BMC Infect Dis. 2016 Jun 2;16(1):243.
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METHODS: The Respiratory Protection Effectiveness Clinical Trial (ResPECT) is a prospective comparison of respiratory protective equipment to be conducted at multiple U.S. study sites. Healthcare personnel who work in outpatient settings will be cluster-randomized to wear N95 respirators or medical masks for protection against infections during respiratory virus season. Outcome measures will include laboratory-confirmed viral respiratory infections, acute respiratory illness, and influenza-like illness. Participant exposures to patients, coworkers, and others with symptoms and signs of respiratory infection, both within and beyond the workplace, will be recorded in daily diaries. Adherence to study protocols will be monitored by the study team.
DISCUSSION: ResPECT is designed to better understand the extent to which N95s and MMs reduce clinical illness among healthcare personnel. A fully successful study would produce clinically relevant results that help clinician-leaders make reasoned decisions about protection of healthcare personnel against occupationally acquired respiratory infections and prevention of spread within healthcare systems.
TRIAL REGISTRATION: The trial is registered at clinicaltrials.gov, number NCT01249625 (11/29/2010).
KEYWORDS: Healthcare personnel; Influenza; Masks; Respirators; Respiratory infections
REFERENCE:
Radonovich LJ Jr, et al. The Respiratory Protection Effectiveness Clinical Trial (ResPECT): a cluster-randomized comparison of respirator and medical mask effectiveness against respiratory infections in healthcare personnel. BMC Infect Dis. 2016 Jun 2;16(1):243.
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lunes, 20 de junio de 2016
LINEAMIENTOS PARA LA GESTIÓN DE RIESGO BIOLÓGICO
Introducción:
La gestión de riesgo biológico es fundamental para la Asociación Mexicana de Bioseguridad A.C., por lo cual se acordó en Junio de 2014, elaborar estos lineamientos, basados en el contenido del documento CWA15793:2011: Laboratory Biorisk Management. Aunque se mantuvo la estructura básica del CWA15793, estos lineamientos se distinguen en múltiples aspectos. Además se ha verificado que se respete la propiedad intelectual del Comité Europeo de Normalización sobre el CWA15793:2011 en su versión en lengua española UNE-CWA 15793:2013).
La AMEXBIO pone a CONSULTA PÚBLICA su BORRADOR DE LOS LINEAMIENTOS PARA LA GESTIÓN DE RIESGO BIOLÓGICO (PDF). Se recibirán comentarios del 25 de mayo al 9 de julio de 2016 en el formato designado únicamente. ¡Gracias por el apoyo!
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La gestión de riesgo biológico es fundamental para la Asociación Mexicana de Bioseguridad A.C., por lo cual se acordó en Junio de 2014, elaborar estos lineamientos, basados en el contenido del documento CWA15793:2011: Laboratory Biorisk Management. Aunque se mantuvo la estructura básica del CWA15793, estos lineamientos se distinguen en múltiples aspectos. Además se ha verificado que se respete la propiedad intelectual del Comité Europeo de Normalización sobre el CWA15793:2011 en su versión en lengua española UNE-CWA 15793:2013).
La AMEXBIO pone a CONSULTA PÚBLICA su BORRADOR DE LOS LINEAMIENTOS PARA LA GESTIÓN DE RIESGO BIOLÓGICO (PDF). Se recibirán comentarios del 25 de mayo al 9 de julio de 2016 en el formato designado únicamente. ¡Gracias por el apoyo!
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