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lunes, 20 de junio de 2016

LINEAMIENTOS PARA LA GESTIÓN DE RIESGO BIOLÓGICO

Introducción:
La gestión de riesgo biológico es fundamental para la Asociación Mexicana de Bioseguridad A.C., por lo cual se acordó en Junio de 2014, elaborar estos lineamientos, basados en el contenido del documento CWA15793:2011: Laboratory Biorisk Management. Aunque se mantuvo la estructura básica del CWA15793, estos lineamientos se distinguen en múltiples aspectos. Además se ha verificado que se respete la propiedad intelectual del Comité Europeo de Normalización sobre el CWA15793:2011 en su versión en lengua española UNE-CWA 15793:2013).

La AMEXBIO pone a CONSULTA PÚBLICA su BORRADOR DE LOS LINEAMIENTOS PARA LA GESTIÓN DE RIESGO BIOLÓGICO (PDF). Se recibirán comentarios del 25 de mayo al 9 de julio de 2016 en el formato designado únicamente.  ¡Gracias por el apoyo!

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martes, 31 de mayo de 2016

HISTORY 1968: Microbiological Studies on the Performance of a Laminar Airflow Biological Cabinet

Engineering and microbiological tests indicated that a typical, commercial laminar airflow cabinet was not effective in providing either product protection or agent containment. The cabinet was modified and tested through a series of alternate configurations to establish a set of design criteria. A mock-up cabinet was developed from these design criteria. The mock-up unit was evaluated for efficiency in providing both product protection and agent containment. In these evaluations, challenge methods were developed to simulate normal, in-use laboratory operations. Controlled bacterial or viral aerosol challenges were used at higher than normal levels to provide stringent test conditions. Test results indicated that the mock-up unit was considerably better in preventing agent penetration (0.1 to 0.2 particles per 100 ft3 of air) than the commercial cabinet (5 to 6 particles per 100 ft3 of air) during product protection tests. Similarly, agent containment was considerably better in the new cabinet (particle escape of 2 to 3 per 100 ft3 of air at only one of the five test sites) than in the commercial cabinet (particle escape of 2 to 14 per 100 ft3 of air at three of the five test sites).

REFERENCE:
Mcdade, Joseph J. et al. “Microbiological Studies on the Performance of a Laminar Airflow Biological Cabinet.” Applied Microbiology 16.7 (1968): 1086–1092. Print.

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lunes, 23 de mayo de 2016

MAPA: Sede del 8º Simposio de Bioseguridad #SIBB16


Todos los cursos y eventos del 8º Simposio Internacional de Bioseguridad y Biocustodia 2016 (SIBB16) tendrán lugar en las instalaciones del:

Instituto de Diagnóstico y Referencia Epidemiológica
Francisco de P. Miranda 177,
Lomas de Plateros,
01480 Álvaro Obregón,
Cd. de México.

¿Cómo llegar?

1. El Aeropuerto Internacional Benito Juárez recibe cada año a millones de visitantes, y está localizado en la ciudad, y cuenta con servicios de Taxis "Seguros", que podrán llevarlo a cualquier punto de la ciudad.

2. La ciudad cuenta con varias estaciones de autobuses con conexiones a las diferentes ciudades del interior. 

La ciudad de México cuenta con unaRED DE TRANSPORTE DE PASAJEROS, que incluye al Metro,Metrobús, trolebús y innumerables autobuses.
Descargue su mapa delMetroyMetrobús

Información turistica:  mexicocity.gob.mx

Se recomienda llegar en transporte público, ya que no hay estacionamientos en un radio de 2 km.

HOTEL SEDE "ONE"

La tarifa ya se encuentra disponible llamando al 018005045000, al 53266900 o bien ingresando a www.onehoteles.com

CLAVE DE DESCUENTO:  Nombre: SIBB2016  Clave: G14FJ8@OPT
El número de habitaciones es limitado. Las primeras reservaciones son las que obtienen el descuento.​

TARIFA:
$900 más impuestos en ocupación sencilla
$1,020 más impuestos en ocupación doble
Incluye:Desayuno, Internet y Llamadas locales

DIRECCIÓN:
Av. Patriotismo No. 229 
Col. San Pedro de los Pinos
03800
Mexico City
Distrito Federal, México

OTRAS OPCIONES DE HOTEL

Opción 2.- Marriot Courtyard 
Dirección:Av. Revolución 333, San Pedro de los Pinos, 11870 Ciudad de México. 
Teléfono:01 55 5627 0220 

Opción 3.- Holiday Inn 
Dirección:Av. Revolución 583, San Pedro de los Pinos, 03800 Ciudad de México. 
Teléfono:01 55 5278 9950 




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Examen de certificación en gestión de riesgo #IFBA

English version,  scroll down. 
PUBLICADO EL 26 DE ABRIL DE 2016
Asegura tu lugar.  
Inscríbete antes del 8 DE JUNIO!
La Asociación Mexicana de Bioseguridad (AMEXBIO) se complace en colaborar con la Federación Internacional de Asociaciones de Bioseguridad (IFBA) en la aplicación de su Examen para la Certificación Profesional en Gestión de Riesgo Biológico durante nuestra próxima conferencia de junio de 2016. Además de ofrecer la Certificación Profesional en Gestión de Riesgos Biológicos, también vamos a ofrecer la nueva Certificación Profesional en Gestión de Residuos Biológicos. Tenga en cuenta que sólo aquellos que han completado con éxito la certificación en Gestión de Riesgos Biológicos son elegibles para esta nueva certificación.
Más detalles sobre el 8º Simposio Internacional de Bioseguridad y Biocustodia de AMEXBIO se pueden encontrar en http://www.amexbio.wildapricot.org/SIBB
La certificación profesional de la IFBA identifica a individuos con competencias demostradas en los principios y prácticas fundamentales en la gestión de riesgos biológicos. Esta es una gran oportunidad para que nuestros colegas puedan avanzar en su carrera y lograr reconocimiento internacional.
Más detalles sobre el programa de certificación se pueden encontrar AQUI. Pueden presentar el examen personas de cualquier país que cumplan los requisitos y se registren en línea. Las preguntas del examen y todos sus materiales están en idioma Inglés. La sesión de examen se llevará a cabo el jueves 16 de junio a las 2:00 pm en el segundo piso Sala de reuniones, Instituto de Diagnóstico y Referencia Epidemiológicos (INDRE) en la Ciudad de México. El examen requiere de registro previo. Todas las solicitudes deben ser ingresadas a través del sistema Certifior en https://ifba.certifior.com. En estas INSTRUCCIONES se explica el proceso de solicitud. Para obtener información sobre la guía de estudio, el contenido del examen y el tipo de preguntas, haga clic GUIA DE ESTUDIO 

Los usuarios de computadoras Mac, se recomienda utilizar navegador Chrome o Firefox durante el registro.
.
Para más información y consultas sobre esta sesión por favor póngase en contacto (en inglés) con la Secretaría de IFBA por correo electrónico a: secretariat@internationalbiosafety.org.
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ENGLISH
The Mexican Biosafety Association (AMEXBIO) is pleased to collaborate with the International Federation of Biosafety Associations in the delivery of the IFBA’s Professional Certification in Biorisk Management examination during our upcoming June conference. Further details on the AMEXBIO’s 7th International Symposium can be found at http://www.amexbio.wildapricot.org/SIBB  
The IFBA’s professional certification identifies individuals with demonstrated competencies in the fundamental principles & practices of biorisk management. This is an exciting opportunity for our members to advance their careers and achieve international recognition among colleagues. Further details on the certification program can be found HERE.
The exam questions and all its materials are in English language. The exam session will be held on Saturday June 16 at the Instituto de Diagnóstico y Referencia Epidemiológica in Mexico City. All applications must be processed through the on-line Certifior system at https://ifba.certifior.comThese instructions will guide individuals through the application process. For information on the exam content and sample questions, click here.  

For further details and enquiries on this session please contact the IFBA Secretariat by email at secretariat@internationalbiosafety.org.


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N95 respirator use during advanced pregnancy

Background: To determine the physiological and subjective effects of wearing an N95 filtering facepiece respirator (N95 FFR) in advanced stages of pregnancy.
Methods: Healthy pregnant women (n = 22) and nonpregnant women (n = 22) had physiological and subjective measurements taken with and without wearing an N95 FFR during exercise and postural sedentary activities over a 1-hour period.
Results: There were no differences between the pregnant and nonpregnant women with respect to heart rate, respiratory rate, oxygen saturation, transcutaneous carbon dioxide level, chest wall temperature, aural temperature, and subjective perceptions of exertion and thermal comfort. No significant effect on fetal heart rate was noted.
Conclusions: Healthy pregnant women wearing an N95 FFR for 1 hour during exercise and sedentary activities did not exhibit any significant differences in measured physiological and subjective responses compared with nonpregnant women.
Keywords: Pregnancy, Respiratory protective equipment, Physiological response, Subjective response, Fetal heart rate

REFERENCE:
Roberge, RJ, et al. “N95 Respirator Use during Advanced Pregnancy.” American journal of infection control 42.10 (2014): 1097–1100. PMC. Web. 16 Apr. 2016.

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viernes, 20 de mayo de 2016

Review: Space microbiology

The responses of microorganisms (viruses, bacterial cells, bacterial and fungal spores, and lichens) to selected factors of space (microgravity, galactic cosmic radiation, solar UV radiation, and space vacuum) were determined in space and laboratory simulation experiments. In general, microorganisms tend to thrive in the space flight environment in terms of enhanced growth parameters and a demonstrated ability to proliferate in the presence of normally inhibitory levels of antibiotics. The mechanisms responsible for the observed biological responses, however, are not yet fully understood. A hypothesized interaction of microgravity with radiation-induced DNA repair processes was experimentally refuted. The survival of microorganisms in outer space was investigated to tackle questions on the upper boundary of the biosphere and on the likelihood of interplanetary transport of microorganisms. It was found that extraterrestrial solar UV radiation was the most deleterious factor of space. Among all organisms tested, only lichens (Rhizocarpon geographicum and Xanthoria elegans) maintained full viability after 2 weeks in outer space, whereas all other test systems were inactivated by orders of magnitude. Using optical filters and spores of Bacillus subtilis as a biological UV dosimeter, it was found that the current ozone layer reduces the biological effectiveness of solar UV by 3 orders of magnitude. If shielded against solar UV, spores of B. subtilis were capable of surviving in space for up to 6 years, especially if embedded in clay or meteorite powder (artificial meteorites). The data support the likelihood of interplanetary transfer of microorganisms within meteorites, the so-called lithopanspermia hypothesis.

REFERENCE:
Horneck G, et al. Space microbiology. Microbiol Mol Biol Rev. 2010 Mar;74(1):121-56. doi: 10.1128/MMBR.00016-09. Review.

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lunes, 16 de mayo de 2016

Exhaled Air Dispersion during Coughing w/wo Wearing a Surgical or N95 Mask

      Room ventilation design and experimental set-up.
Objectives: We compared the expelled air dispersion distances during coughing from a human patient simulator (HPS) lying at 45° with and without wearing a surgical mask or N95 mask in a negative pressure isolation room.
Methods: Airflow was marked with intrapulmonary smoke. Coughing bouts were generated by short bursts of oxygen flow at 650, 320, and 220L/min to simulate normal, mild and poor coughing efforts, respectively. The coughing jet was revealed by laser light-sheet and images were captured by high definition video. Smoke concentration in the plume was estimated from the light scattered by smoke particles. Significant exposure was arbitrarily defined where there was ≥ 20% of normalized smoke concentration.
Results: During normal cough, expelled air dispersion distances were 68, 30 and 15 cm along the median sagittal plane when the HPS wore no mask, a surgical mask and a N95 mask, respectively. In moderate lung injury, the corresponding air dispersion distances for mild coughing efforts were reduced to 55, 27 and 14 cm, respectively, p < 0.001. The distances were reduced to 30, 24 and 12 cm, respectively during poor coughing effort as in severe lung injury. Lateral dispersion distances during normal cough were 0, 28 and 15 cm when the HPS wore no mask, a surgical mask and a N95 mask, respectively.
Conclusions: Normal cough produced a turbulent jet about 0.7 m towards the end of the bed from the recumbent subject. N95 mask was more effective than surgical mask in preventing expelled air leakage during coughing but there was still significant sideway leakage.

REFERENCE:
Hui, David S. et al. “Exhaled Air Dispersion during Coughing with and without Wearing a Surgical or N95 Mask.” Ed. Ravi Jhaveri. PLoS ONE 7.12 (2012): e50845. PMC. Web. 16 Apr. 2016.

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lunes, 9 de mayo de 2016

Bactericidal Effects and Mechanism of Action of Olanexidine Gluconate, a New Antiseptic.

Chemical structures of olanexidine (A)
and chlorhexidine (B).
Olanexidine gluconate [1-(3,4-dichlorobenzyl)-5-octylbiguanide gluconate] (development code OPB-2045G) is a new monobiguanide compound with bactericidal activity. In this study, we assessed its spectrum of bactericidal activity and mechanism of action. The minimal bactericidal concentrations of the compound for 30-, 60-, and 180-s exposures were determined with the microdilution method using a neutralizer against 320 bacterial strains from culture collections and clinical isolates. Based on the results, the estimated bactericidal olanexidine concentrations with 180-s exposures were 869 μg/ml for Gram-positive cocci (155 strains), 109 μg/ml for Gram-positive bacilli (29 strains), and 434 μg/ml for Gram-negative bacteria (136 strains). Olanexidine was active against a wide range of bacteria, especially Gram-positive cocci, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci, and had a spectrum of bactericidal activity comparable to that of commercial antiseptics, such as chlorhexidine and povidone-iodine. In vitro experiments exploring its mechanism of action indicated that olanexidine (i) interacts with the bacterial surface molecules, such as lipopolysaccharide and lipoteichoic acid, (ii) disrupts the cell membranes of liposomes, which are artificial bacterial membrane models, (iii) enhances the membrane permeability of Escherichia coli, (iv) disrupts the membrane integrity of S. aureus, and (v) denatures proteins at relatively high concentrations (≥160 μg/ml). These results indicate that olanexidine probably binds to the cell membrane, disrupts membrane integrity, and its bacteriostatic and bactericidal effects are caused by irreversible leakage of intracellular components. At relatively high concentrations, olanexidine aggregates cells by denaturing proteins. This mechanism differs slightly from that of a similar biguanide compound, chlorhexidine.

Hagi A, et al. Bactericidal Effects and Mechanism of Action of Olanexidine Gluconate, a New Antiseptic. Antimicrob Agents Chemother. 2015 Aug;59(8):4551-9. doi: 10.1128/AAC.05048-14. Epub 2015 May 18.

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jueves, 5 de mayo de 2016

HISTORY 1968: Containment of Microbial Aerosols in a Microbiological Safety Cabinet

A microbiological safety cabinet was evaluated to determine conditions under which microorganisms might escape. Tests were conducted under three cabinet-closure conditions, various airflow velocities, and different laboratory operations, with 105, 1.1 × 105, and 106 microorganisms per cubic foot of cabinet space released per min for 5 min. The data revealed that (i) escape of a human infectious dose is possible when the cabinet is used with the glove panel off; (ii) the number of organisms that escaped from the cabinet increased with a decrease in air velocity; and (iii) an increase in the number of laboratory operations resulted in an increase in the number of organisms that escaped. Thus, when the glove panel was off, the cabinet was only safe for operations that released a small number of microorganisms into the cabinet, whereas the cabinet was safe for operations of significantly greater hazard when used with the glove panel on but with the gloves unattached.

REFERENCE:
Barbeito, Manuel S., and Larry A. Taylor. “Containment of Microbial Aerosols in a Microbiological Safety Cabinet.” Applied Microbiology 16.8 (1968): 1225–1229. Print.

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lunes, 2 de mayo de 2016

Respiratory consequences of N95-type Mask usage in pregnant healthcare workers-a controlled clinical study.

Tight fitting Hans Rudolph respirator masks
used in Phase II. (
a) Control cycles with outlet
open to air, and (
b) N95 cycles with outlet
covered by N95 mask materials
BACKGROUND: Outbreaks of emerging infectious diseases have led to guidelines recommending the routine use of N95 respirators for healthcare workers, many of whom are women of childbearing age. The respiratory effects of prolonged respirator use on pregnant women are unclear although there has been no definite evidence of harm from past use.
METHODS: We conducted a two-phase controlled clinical study on healthy pregnant women between 27 to 32 weeks gestation. In phase I, energy expenditure corresponding to the workload of routine nursing tasks was determined. In phase II, pulmonary function of 20 subjects was measured whilst at rest and exercising to the predetermined workload while breathing ambient air first, then breathing through N95-mask materials.
RESULTS: Exercising at 3 MET while breathing through N95-mask materials reduced mean tidal volume (TV) by 23.0 % (95 % CI -33.5 % to -10.5 %, p < 0.001) and lowered minute ventilation (VE) by 25.8 % (95 % CI -34.2 % to -15.8 %, p < 0.001), with no significant change in breathing frequency compared to breathing ambient air. Volumes of oxygen consumption (VO2) and carbon dioxide expired (VCO2) were also significantly reduced; VO2 by 13.8 % (95 % CI -24.2 % to -3 %, p = 0.013) and VCO2 by 17.7 %, (95 % CI -28.1 % to -8.6 %, p = 0.001). Although no changes in the inspired oxygen and carbon dioxide concentrations were demonstrated, breathing through N95-mask materials during low intensity work (3 MET) reduced expired oxygen concentration by 3.2 % (95 % CI: -4.1 % to -2.2 %, p < 0.001), and increased expired carbon dioxide by 8.9 % (95 % CI: 6.9 % to 13.1 %; p <0.001) suggesting an increase in metabolism. There were however no changes in the maternal and fetal heart rates, finger-tip capillary lactate levels and oxygen saturation and rating of perceived exertion at the work intensity investigated.
CONCLUSIONS: Breathing through N95 mask materials have been shown to impede gaseous exchange and impose an additional workload on the metabolic system of pregnant healthcare workers, and this needs to be taken into consideration in guidelines for respirator use. The benefits of using N95 mask to prevent serious emerging infectious diseases should be weighed against potential respiratory consequences associated with extended N95 respirator usage.

REFERENCE:
Tong PS, et al. Respiratory consequences of N95-type Mask usage in pregnant healthcare workers-a controlled clinical study. Antimicrob Resist Infect Control. 2015 Nov 16;4:48. doi: 10.1186/s13756-015-0086-z. eCollection 2015.

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