Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the necessary foresight, and these Guidelines have been prepared to provide those new to the field and others engaged in teaching and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them effectively. The Guidelines cover areas such as development, acquisition, authentication, cryopreservation, transfer of cell lines between laboratories, microbial contamination, characterisation, instability and misidentification. Advice is also given on complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection and maintenance of equipment and how to deal with problems that may arise.
Keywords: cell culture, mycoplasma contamination, Human Tissue Act, cell line, cell line misidentification, cryostorage, Human Tissue Authority, STR profiling, human tissue, Human Fertilisation and Embryology Act
REFERENCE:
Geraghty, R J et al. “
Guidelines for the Use of Cell Lines in Biomedical Research.” British Journal of Cancer 111.6 (2014): 1021–1046. PMC. Web. 9 July 2015.
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