Installing biosafety level 3 containment laboratories in low- and middle-income countries

In Mali, the incidence of tuberculosis (TB) is estimated at 56 cases per 100 000 people, with a prevalence of multidrug-resistant TB in new cases of 1.7% (range, 0.3-3.1%) and in retreatment cases of 17% (range, 4.4-30%). Appropriate biosafety conditions for performing routine TB culture and antimicrobial susceptibility testing have been lacking. In 2015, a biosafety level 3 (BSL3) laboratory set up in a shipping container was donated to the Malian Ministry of Health and Public Hygiene to provide capacity for TB testing. This laboratory is now managed by Malian laboratory staff and is processing samples at the national level. We explain the necessary steps for establishing and running a BSL3 laboratory. Despite the acute need for functioning and sustainable BSL3 laboratories, low- and middle-income countries are faced with a complex process and must overcome many challenges.
REFERENCE:
Kouriba B, Ouwe Missi Oukem-Boyer O, Traoré B, Touré A, Raskine L, Babin FX. Installing biosafety level 3 containment laboratories in low- and middle-income countries: challenges and prospects from Mali's experience. New Microbes New Infect. 2018 Jun 20;26:S74-S77. doi: 10.1016/j.nmni.2018.05.011.

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